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software, algorithm prism 9  (GraphPad Software Inc)


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    Structured Review

    GraphPad Software Inc software, algorithm prism 9
    Software, Algorithm Prism 9, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software%2C+algorithm+prism+9/software++algorithm+prism+9/10__7554_slash_elife__101043-161-181-184
    Average 90 stars, based on 1 article reviews
    software, algorithm prism 9 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) Hela- N Maureen Powers Lab HeLa cells selected for transfectability Cell line (Homo sapiens) HEK293T ATCC CRL- 3216 Antibody Anti- FLAG (M2, mouse monoclonal) Sigma F1804 WB (1:5000) Antibody Anti- MYC (4A6, mouse monoclonal) Millipore 05- 724 WB (1:2500) Antibody Anti- RFP (rat monoclonal) ChromoTek 5F8 IF (1:500) Antibody Anti- RFP (mouse monoclonal) ChromoTek 6G6 WB (1:1000) Antibody Anti- alpha- tubulin (DM1A, mouse monoclonal) Cell Signaling Technologies DM1A IF (1:2000) Antibody Anti- alpha- Tubulin (chicken polyclonal) Synaptic Systems 302 206 IF (1:1000) Antibody Anti- CAPN7 (rabbit polyclonal) Proteintech Cat# 26985- 1- AP IF (1:500) WB (1:4000) Antibody Anti- IST1 (rabbit polyclonal) Sundquist Lab/Covance UT560 IF (1:1000) Paine et al. eLife 2023;12:e84515. .. DOI: https://doi.org/10.7554/eLife.84515 15 of 25 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- NUP153 (SA1) (mouse monoclonal) Brian Burke WB (1:50) Antibody Anti- NUP50 (rabbit polyclonal) Mackay et al., 2010 WB (1:2500) Antibody Anti- GAPDH (mouse monoclonal) Millipore Sequence- based reagent siNT Mackay et al., 2010 siRNA GCAAAUCUCCGAUCGUAGA Sequence- based reagent siCAPN7 Wenzel et al., 2022 siRNA GCACCCAUACCUUUACAUU Sequence- based reagent siNUP153 Mackay et al., 2010 siRNA GGACUUGUUAGAUCUAGUU Chemical compound, drug Doxycycline Hyclate Sigma 324385 1–2 μg/mL Chemical compound, drug Thymidine CalBiochem CAS 50- 89- 5 2 mM Chemical compound, drug Oregon Green 488 maleimide Life Technologies/Molecular Probes O6034 Fluorescent label for peptides Software, algorithm Fiji NIH RRID:SCR_002285 Software, algorithm Prism 9 GraphPad Continued .. All plasmids created for this study were made by Gibson Assembly (Gibson et al., 2009) using vectors linearized by restriction enzymes (New England Biolabs). pCA528 bacterial expression plasmids expressing His6- SUMO- tagged fusion proteins with a native N- terminus after tag cleavage were linearized with BsaI and BamHI. pCAG- OSF mammalian expression plasmids that encoded proteins for co- IP binding assays were linearized with KpnI and XhoI. pLVX vectors used for generating inducible cell lines were linearized by BamHI and MluI.

    Sequencing:

    Article Title: The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) Hela- N Maureen Powers Lab HeLa cells selected for transfectability Cell line (Homo sapiens) HEK293T ATCC CRL- 3216 Antibody Anti- FLAG (M2, mouse monoclonal) Sigma F1804 WB (1:5000) Antibody Anti- MYC (4A6, mouse monoclonal) Millipore 05- 724 WB (1:2500) Antibody Anti- RFP (rat monoclonal) ChromoTek 5F8 IF (1:500) Antibody Anti- RFP (mouse monoclonal) ChromoTek 6G6 WB (1:1000) Antibody Anti- alpha- tubulin (DM1A, mouse monoclonal) Cell Signaling Technologies DM1A IF (1:2000) Antibody Anti- alpha- Tubulin (chicken polyclonal) Synaptic Systems 302 206 IF (1:1000) Antibody Anti- CAPN7 (rabbit polyclonal) Proteintech Cat# 26985- 1- AP IF (1:500) WB (1:4000) Antibody Anti- IST1 (rabbit polyclonal) Sundquist Lab/Covance UT560 IF (1:1000) Paine et al. eLife 2023;12:e84515. .. DOI: https://doi.org/10.7554/eLife.84515 15 of 25 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- NUP153 (SA1) (mouse monoclonal) Brian Burke WB (1:50) Antibody Anti- NUP50 (rabbit polyclonal) Mackay et al., 2010 WB (1:2500) Antibody Anti- GAPDH (mouse monoclonal) Millipore Sequence- based reagent siNT Mackay et al., 2010 siRNA GCAAAUCUCCGAUCGUAGA Sequence- based reagent siCAPN7 Wenzel et al., 2022 siRNA GCACCCAUACCUUUACAUU Sequence- based reagent siNUP153 Mackay et al., 2010 siRNA GGACUUGUUAGAUCUAGUU Chemical compound, drug Doxycycline Hyclate Sigma 324385 1–2 μg/mL Chemical compound, drug Thymidine CalBiochem CAS 50- 89- 5 2 mM Chemical compound, drug Oregon Green 488 maleimide Life Technologies/Molecular Probes O6034 Fluorescent label for peptides Software, algorithm Fiji NIH RRID:SCR_002285 Software, algorithm Prism 9 GraphPad Continued .. All plasmids created for this study were made by Gibson Assembly (Gibson et al., 2009) using vectors linearized by restriction enzymes (New England Biolabs). pCA528 bacterial expression plasmids expressing His6- SUMO- tagged fusion proteins with a native N- terminus after tag cleavage were linearized with BsaI and BamHI. pCAG- OSF mammalian expression plasmids that encoded proteins for co- IP binding assays were linearized with KpnI and XhoI. pLVX vectors used for generating inducible cell lines were linearized by BamHI and MluI.

    Software:

    Article Title: The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) Hela- N Maureen Powers Lab HeLa cells selected for transfectability Cell line (Homo sapiens) HEK293T ATCC CRL- 3216 Antibody Anti- FLAG (M2, mouse monoclonal) Sigma F1804 WB (1:5000) Antibody Anti- MYC (4A6, mouse monoclonal) Millipore 05- 724 WB (1:2500) Antibody Anti- RFP (rat monoclonal) ChromoTek 5F8 IF (1:500) Antibody Anti- RFP (mouse monoclonal) ChromoTek 6G6 WB (1:1000) Antibody Anti- alpha- tubulin (DM1A, mouse monoclonal) Cell Signaling Technologies DM1A IF (1:2000) Antibody Anti- alpha- Tubulin (chicken polyclonal) Synaptic Systems 302 206 IF (1:1000) Antibody Anti- CAPN7 (rabbit polyclonal) Proteintech Cat# 26985- 1- AP IF (1:500) WB (1:4000) Antibody Anti- IST1 (rabbit polyclonal) Sundquist Lab/Covance UT560 IF (1:1000) Paine et al. eLife 2023;12:e84515. .. DOI: https://doi.org/10.7554/eLife.84515 15 of 25 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- NUP153 (SA1) (mouse monoclonal) Brian Burke WB (1:50) Antibody Anti- NUP50 (rabbit polyclonal) Mackay et al., 2010 WB (1:2500) Antibody Anti- GAPDH (mouse monoclonal) Millipore Sequence- based reagent siNT Mackay et al., 2010 siRNA GCAAAUCUCCGAUCGUAGA Sequence- based reagent siCAPN7 Wenzel et al., 2022 siRNA GCACCCAUACCUUUACAUU Sequence- based reagent siNUP153 Mackay et al., 2010 siRNA GGACUUGUUAGAUCUAGUU Chemical compound, drug Doxycycline Hyclate Sigma 324385 1–2 μg/mL Chemical compound, drug Thymidine CalBiochem CAS 50- 89- 5 2 mM Chemical compound, drug Oregon Green 488 maleimide Life Technologies/Molecular Probes O6034 Fluorescent label for peptides Software, algorithm Fiji NIH RRID:SCR_002285 Software, algorithm Prism 9 GraphPad Continued .. All plasmids created for this study were made by Gibson Assembly (Gibson et al., 2009) using vectors linearized by restriction enzymes (New England Biolabs). pCA528 bacterial expression plasmids expressing His6- SUMO- tagged fusion proteins with a native N- terminus after tag cleavage were linearized with BsaI and BamHI. pCAG- OSF mammalian expression plasmids that encoded proteins for co- IP binding assays were linearized with KpnI and XhoI. pLVX vectors used for generating inducible cell lines were linearized by BamHI and MluI.

    other:

    Article Title: Synaptic cell adhesion molecule Cdh6 identifies a class of sensory neurons with novel functions in colonic motility
    Article Snippet: resource Designation Source or reference Identifiers Additional information Antibody Donkey anti- sheep AF- 488 (polyclonal) Invitrogen #A11015, RRID:AB_141362 IF(1:1000) Antibody Donkey anti- rabbit AF- 488 (polyclonal) Invitrogen #A21206, RRID:AB_2535792 IF(1:1000) Peptide, recombinant protein Streptavidin AF- 546 Invitrogen #S11225 IF(1:500) Sequence- based reagent RNAscope probe Mm- Cdh6 Advanced Cell Diagnostics Cat #519541 Sequence- based reagent RNAscope probe Mm- Cdh8 Advanced Cell Diagnostics Cat #485461 Sequence- based reagent RNAscope probe Mm- Nmu Advanced Cell Diagnostics Cat #446831 Sequence- based reagent RNAscope probe Mm- Calcb Advanced Cell Diagnostics Cat #425511 Sequence- based reagent RNAscope probe Mm- eGFP Advanced Cell Diagnostics Cat #400281 Commercial assay or kit RNAscope Multiplex Fluorescent V2 Assay kit with RNA- Protein Co- detection Ancillary Kit Advanced Cell Diagnostics Cat #323100 Chemical compound, drug Protease XIV Sigma #P5417 Chemical compound, drug Collagenase Worthington #CLS- 4 Chemical compound, drug Dispase Sigma #D4693 Chemical compound, drug ZD7288 Sigma- Aldrich #73777 Chemical compound, drug Cesium chloride Sigma- Aldrich #C4036 Chemical compound, drug Tetrodotoxin citrate Alomone Labs #T- 550 Software, algorithm Imaris Filament Tracer Bitplane, Oxford Instruments Software, algorithm LabChart 7, 8 AD Instruments Software, algorithm Prism 9 GraphPad Continued



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